IMMUNOHISTOCHEMICAL ANALYSIS OF EPITHELIAL-MESENCHYMAL TRANSITION BIOMARKERS IN ADENOID CYSTIC CARCINOMA

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology Vol. 137; no. 6; pp. e280 - e281
• Main Authors: COSTA, Carla Samily de Oliveira, ROLIM, Larissa Santos Amaral, MAFRA, Rodrigo Porpino, de SOUZA, Lélia Batista, SQUARIZE, Cristiane Helena, CASTILHO, Rogério Moraes, PINTO, Leão Pereira
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.06.2024
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2023.12.631

To evaluate the immunoexpression of the epithelial-mesenchymal transition biomarkers (Twist1, Snail1, Vimentin, and E-cadherin) in specimens of adenoid cystic carcinoma (ACC), and to investigate possible correlations between them. Twenty cases of ACC were selected for quantitative and semi-quantitative immunohistochemical analysis and by cellular compartment. To assess survival, association with clinicopathological parameters, and histopathological grading systems, the cases were categorized into low and high expression. The immunohistochemical analysis revealed significant differences between the immunoexpression of Vimentin when compared with the clinical variables: the presence of lymph node metastasis, distant metastasis, late clinical staging, and death (p<0.05). In addition, a significant difference was found between the cytoplasmic immunoexpression of Snail1 with the non-occurrence of vascular invasion (p<0.05). There were no significant differences between clinicopathological parameters and grading systems, and no significant associations between immunohistochemical variables and specific survival and disease-free survival (p>0.05). Regarding the correlation test, there was an inversely proportional correlation between the cytoplasmic expression of Twist1 and Vimentin (p<0.05). In this study, it was possible to conclude that epithelial-mesenchymal transition biomarkers are significantly expressed in ACC specimens, possibly associated with their development process, thus highlighting the role of vimentin. Financial support: CNPq and CAPES.