Umbelliprenin-coated Fe 3O 4 magnetite nanoparticles: Antiproliferation evaluation on human Fibrosarcoma cell line (HT-1080)
The potential applications of Fe 3O 4 magnetite nanoparticles (MNPs) in nanomedicine as drug delivery systems are well known. In this study we prepared umbelliprenin-coated Fe 3O 4 MNPs and evaluated the antiproliferative effect of combination in vitro. After synthesis of Fe 3O 4 MNPs, particles wer...
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Published in | Materials Science & Engineering C Vol. 30; no. 7; pp. 1038 - 1042 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
2010
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Subjects | |
Online Access | Get full text |
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Summary: | The potential applications of Fe
3O
4 magnetite nanoparticles (MNPs) in nanomedicine as drug delivery systems are well known. In this study we prepared umbelliprenin-coated Fe
3O
4 MNPs and evaluated the antiproliferative effect of combination
in vitro. After synthesis of Fe
3O
4 MNPs, particles were characterized by transmission electron microscopy, energy-dispersive spectroscopy, and X-ray diffraction spectroscopy techniques. The natural candidate compound — umbelliprenin— was isolated and identified and umbelliprenin-coated Fe
3O
4 MNPs were prepared, using precipitation method. The surface chemistry of umbelliprenin-coated Fe
3O
4 MNPs as well as their thermal decomposition characteristics was examined using Fourier transform infrared spectroscopy and Thermogravimetric Analyzer equipment, respectively. HT-1080 cells were cultured until the logarithmic phase of growth, and MTT assay was successfully carried out to evaluate the possible cytotoxic effects of umbelliprenin-coated Fe
3O
4 MNPs in viable cells
in vitro. The results demonstrated that umbelliprenin has moderate antiproliferative effects with IC
50 value of 50
µg/mL. However, the combination of umbelliprenin and Fe
3O
4 MNPs showed the IC
50 value of 9
µg/mL. In other words, cell proliferation decreased to the remarkably-low proportion of 45% after treating cells with umbelliprenin-coated Fe
3O
4 MNPs. This suggests that with the aid of nanoparticles as carriers, natural products may have even broader range of medical applications in future. |
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ISSN: | 0928-4931 1873-0191 |
DOI: | 10.1016/j.msec.2010.05.005 |