Dissemination of multidrug-resistant Acinetobacter baumannii genotypes carrying bla OXA-23 collected from hospitals in Rio de Janeiro, Brazil

The present study reports the dissemination of multidrug-resistant (MDR) OXA-23-producing Acinetobacter baumannii clones throughout hospitals in Rio de Janeiro, Brazil. A total of 110 imipenem-resistant A. baumannii isolates were obtained from January 2006 to September 2007 in eight hospitals. The m...

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Bibliographic Details
Published inInternational journal of antimicrobial agents Vol. 34; no. 1; pp. 25 - 28
Main Authors Carvalho, Karyne Rangel, Carvalho-Assef, Ana Paula D’Alincourt, Peirano, Gisele, Santos, Lia Cristina Galvão dos, Pereira, Maria José Felix, Asensi, Marise Dutra
Format Journal Article
LanguageEnglish
Published Elsevier B.V 2009
Subjects
Acinetobacter baumannii
Molecular typing
Multidrug resistance
OXA-23
Acinetobacter baumannii
Molecular typing
OXA-23
Multidrug resistance
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Summary:The present study reports the dissemination of multidrug-resistant (MDR) OXA-23-producing Acinetobacter baumannii clones throughout hospitals in Rio de Janeiro, Brazil. A total of 110 imipenem-resistant A. baumannii isolates were obtained from January 2006 to September 2007 in eight hospitals. The modified Hodge test was performed to screen for carbapenemase production. Polymerase chain reaction (PCR) and DNA sequencing were performed for the detection of bla IMP, bla VIM, bla OXA-23-like, bla OXA-24-like, bla OXA-58 and the class 1 integron. Isolates were typed by pulsed-field gel electrophoresis (PFGE) following digestion with ApaI. All the isolates were MDR and 96 (87.3%) produced the carbapenemase OXA-23. No isolates produced OXA-24, OXA-58 or the metallo-β-lactamases IMP and VIM. The class 1 integron was absent in all isolates. The A. baumannii isolates were separated into five genotypes, with the highest prevalence of genotype A (71.8%) followed by genotype B (22.7%). Genotype A was present in seven hospitals, whilst genotype B had spread in five hospitals. The OXA-23-producing isolates belonged to all genotypes. The presence of MDR OXA-23-producing A. baumannii in different hospitals in Rio de Janeiro emphasises the need to control the use of carbapenems and to prevent the spread of these organisms in Rio de Janeiro.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2008.12.009