P 061 - N,N.N′,N′-tetramethylhydroethidine (TMHE) - in search for better probes for the detection of superoxide radical anion

• Published in: Free radical biology & medicine Vol. 108; p. S38
• Main Authors: Sikora, Adam, Michalski, Radosław, Hardy, Micael, Ouari, Olivier, Jan Adamus, Marcinek, Andrzej, Zielonka, Jacek, Kalyanaraman, Balaraman
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.07.2017
• Subjects: hydroethidine; Superoxide radical anion; Superoxide radical anion; hydroethidine
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2017.04.146

Superoxide radical anion is a product of one-electron reduction of molecular oxygen. In vivo superoxide radical anion rapidly reacts with nitric oxide with the formation of peroxynitrite (ONOO−) or undergoes spontaneous/SOD-catalyzed dismutation with the formation of hydrogen peroxide (H2O2) and thus it is a precursor of other biologically relevant oxidants playing an important role in various pathologies. Due to its reactivity and short lifetime, its detection and quantitation is difficult and demands special techniques. One of the approaches is the use of fluorogenic probes, the compounds which themselves are not fluorescent but in the reaction with superoxide radical anion are oxidatively transformed into the fluorescent products that can be directly detected. Among various profluorescent probes available hydroethidine (HE) seems to be a gold standard for detection of superoxide in biological systems. Here we present a spectroscopic and chemical characterization of new analogue of hydroethidine - N,N.N’,N’-tetramethylhydroethidine (TMHE).