Effect of coenzyme Q 10 intake on endogenous coenzyme Q content, mitochondrial electron transport chain, antioxidative defenses, and life span of mice

• Published in: Free radical biology & medicine Vol. 40; no. 3; pp. 480 - 487
• Main Authors: Sohal, Rajindar S., Kamzalov, Sergey, Sumien, Nathalie, Ferguson, Melissa, Rebrin, Igor, Heinrich, Kevin R., Forster, Michael J.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 2006
• Subjects: Aging; Antioxidants; Coenzyme Q; Free radicals; Glutathione; Mitochondria; Oxidative stress; Redox state; Ubiquinone; GSSG; Oxidative stress; Redox state; Free radicals; Coenzyme Q; Antioxidants; Mitochondria; Ubiquinone; ETC; Aging; SMPs; GSH; Glutathione
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2005.08.037

The main purpose of this study was to determine whether intake of coenzyme Q 10, which can potentially act as both an antioxidant and a prooxidant, has an impact on indicators of oxidative stress and the aging process. Mice were fed diets providing daily supplements of 0, 93, or 371 mg CoQ 10 /kg body weight, starting at 3.5 months of age. Effects on mitochondrial superoxide generation, activities of oxidoreductases, protein oxidative damage, glutathione redox state, and life span of male mice were determined. Amounts of CoQ 9 and CoQ 10, measured after 3.5 or 17.5 months of intake, in homogenates and mitochondria of liver, heart, kidney, skeletal muscle, and brain increased with the dosage and duration of CoQ 10 intake in all the tissues except brain. Activities of mitochondrial electron transport chain oxidoreductases, rates of mitochondrial O 2 generation, state 3 respiration, carbonyl content, glutathione redox state of tissues, and activities of superoxide dismutase, catalase, and glutathione peroxidase, determined at 19 or 25 months of age, were unaffected by CoQ 10 administration. Life span studies, conducted on 50 mice in each group, showed that CoQ 10 administration had no effect on mortality. Altogether, the results indicated that contrary to the historical view, supplemental intake of CoQ 10 elevates the endogenous content of both CoQ 9 and CoQ 10, but has no discernable effect on the main antioxidant defenses or prooxidant generation in most tissues, and has no impact on the life span of mice.