Rationally designed an innovative proximity labeling near-infrared fluorogenic probe for imaging of peroxynitrite in acute lung injury

• Published in: Chinese chemical letters
• Main Authors: Tang, Dandan, Xu, Ningge, Fu, Yuyang, Peng, Wei, Wu, Jinsheng, Liu, Heng, Yu, Fabiao
• Format: Journal Article
• Language: English
• Published: Elsevier B.V
• Subjects: Acute lung injury; In situ bioimaging; Near-infrared; Peroxynitrite; Proximity labeling; Acute lung injury; Peroxynitrite; In situ bioimaging; Near-infrared; Proximity labeling
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2024.110082

Acute lung injury (ALI) is a serious clinical condition with a high mortality rate. Oxidative stress and inflammatory responses play pivotal roles in the pathogenesis of ALI. ONOO− is a key mediator that exacerbates oxidative damage and microvascular permeability in ALI. Accurate detection of ONOO− would facilitate early diagnosis and intervention in ALI. Near-infrared fluorescence (NIRF) probes offer new solutions due to their sensitivity, depth of tissue penetration, and imaging capabilities. However, the developed ONOO− fluorescent probes face problems such as interference from other reactive oxygen species and easy intracellular diffusion. To address these issues, we introduced an innovative self-immobilizing NIRF probe, DCI2F-OTf, which was capable of monitoring ONOO−in vitro and in vivo. Importantly, leveraging the high reactivity of the methylene quinone (QM) intermediate, DCI2F-OTf was able to covalently label proteins in the presence of ONOO−, enabling in situ imaging. In mice models of ALI, DCI2F-OTf enabled real-time imaging of ONOO− levels and found that ONOO− was tightly correlated with the progression of ALI. Our findings demonstrated that DCI2F-OTf was a promising chemical tool for the detection of ONOO−, which could help to gain insight into the pathogenesis of ALI and monitor treatment efficacy. An innovative self-immobilizing NIRF probe DCI2F-OTf was capable of monitoring ONOO−in vitro and in vivo. DCI2F-OTf was able to covalently label proteins in the presence of ONOO−, enabling in situ imaging. In mice models of ALI, DCI2F-OTf enabled real-time imaging of ONOO− levels and found that ONOO− was tightly correlated with the progression of ALI. [Display omitted]