Tachyphylaxis to 5-HT 3-receptor-mediated activation of vagal afferents is prevented by co-activation of 5-HT 2 receptors

• Published in: Brain research Vol. 1093; no. 1; pp. 105 - 115
• Main Authors: Lacolley, Patrick J., Owen, Joy R., Bates, James N., Johnson, Alan Kim, Lewis, Stephen J.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2006
• Subjects: 5-HT 3 receptors; Rat; Serotonin (5-HT); Tachyphylaxis; Vagal afferents; Tachyphylaxis; Rat; Serotonin (5-HT); 5-HT 3 receptors; Vagal afferents
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.03.090

Functional studies have provided evidence that 5-HT 3 ion-channel receptors (5-HT 3Rs) on vagal cardiopulmonary afferents mediating the Bezold–Jarisch reflex (BJR) rapidly desensitize upon repeated exposure to selective 5-HT 3R agonists. G-protein-coupled 5-HT 2 receptors (5-HT 2Rs) also exist on vagal afferents, although activation of these receptors does not elicit the BJR. However, there is in vivo evidence that 5-HT 2Rs may regulate the activity of 5-HT 3Rs. The aim of this study was to determine whether co-activation of 5-HT 2Rs prevents desensitization of 5-HT 3Rs mediating the BJR in conscious rats. The principal findings were that (1) tachyphylaxis rapidly developed to the BJR-mediated hemodynamic responses elicited by successive injections of 5-HT 3R agonists and (2) co-injection of the selective 5-HT 2R agonist, α-methyl-5-HT, prevented tachyphylaxis to the BJR-mediated hemodynamic responses elicited by the 5-HT 3R agonists. Additional studies provided evidence that (1) tachyphylaxis to the 5-HT 3R agonists was not due to impairment of the central or efferent processing of the BJR, and (2) the pressor responses elicited by α-methyl-5-HT were not responsible for preventing tachyphylaxis to the BJR reflex responses elicited by 5-HT 3R agonists. These results suggest that the loss of response to 5-HT 3R agonists is due to desensitization of 5-HT 3Rs on vagal afferents mediating the BJR and that co-activation of 5-HT 2Rs prevents the desensitization of these 5-HT 3Rs.