Stem cell attachment to layer-by-layer assembled TiO 2 nanoparticle thin films

• Published in: Biomaterials Vol. 27; no. 24; pp. 4296 - 4303
• Main Authors: Kommireddy, Dinesh S., Sriram, Shashikanth M., Lvov, Yuri M., Mills, David K.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2006
• Subjects: Layer-by-layer assembly; Nanoparticles; Stem cells; Surface modification; Surface roughness; Titanium dioxide; Nanoparticles; Surface modification; Stem cells; Surface roughness; Layer-by-layer assembly; Titanium dioxide
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2006.03.042

Surface topography is one of the most important factors influencing the attachment and spreading of cells. In the present study, layer-by-layer assembled titanium dioxide (TiO 2) nanoparticle thin films were chosen for attachment, proliferation and spreading studies on mouse mesenchymal stem cells (MSC). Increasing surface roughness was observed with increasing number of layer-by-layer assembled TiO 2 thin films. Four layer TiO 2 thin film showed higher number of attached cells than a one layer thin film and control surfaces. MSCs experienced no cytotoxic effects after culture on the TiO 2 coated substrates as observed from the cytotoxicity tests. Cell spreading, visualized with scanning electron microscopy, showed a faster rate of spreading on a rougher surface. Cells on a four-layer substrate, at 12 h showed complete spreading, where as most of the cells on a control surface and a one-layer surface, at 24 h, retained a rounded morphology. In conclusion, TiO 2 nanoparticle thin films were successfully assembled in alternation with polyelectrolytes and in-vitro studies with MSC showed an increase in the attachment and faster spreading of cells on rougher surfaces.