Prostaglandin F 2alpha enhances glucose consumption through neither adipocyte differentiation nor GLUT1 expression in 3T3-L1 cells

• Published in: Cellular signalling Vol. 13; no. 2; pp. 105 - 109
• Main Authors: Kamon, Junji, Naitoh, Takeshi, Kitahara, Masaki, Tsuruzoe, Nobutomo
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 2001
• Subjects: 3T3-L1 cell; Adipocyte differentiation; Glucose transporter; Glucose uptake; PGF 2alpha; Thiazolidinedione; Glucose transporter; PG, prostaglandin; EGTA, ethyleneglycol-bis-(beta-aminoethyl ether) N, N, N′, N′-tetraacetic acid; FBS, fetal bovine serum; DEX, dexamethasone; 3T3-L1 cell; 15d-PGJ 2, 15-deoxy-delta 12,14-prostaglandin J 2; IBMX, 3-isobutyl-1-methyl-xanthine; NADH, dihydronicotinamide adenine dinucleotide; Thiazolidinedione; PPARgamma, peroxisome proliferator-activated receptor gamma; TG, triglyceride; Glucose uptake; GPDH, glycerol-3-phosphate dehydrogenase; AA, arachidonic acid; DMEM, Dulbecco's modified Eagle's medium; TZD, thiazolidinedione; Adipocyte differentiation; PGF 2alpha; cPGI 2, carbacyclin
• ISSN: 0898-6568; 1873-3913
• DOI: 10.1016/S0898-6568(00)00137-6

Arachidonic acid (AA) at 0.2 mM enhances glucose uptake through increased levels of glucose transporter (GLUT) 1 protein in 3T3-L1 adipocytes. Since AA is a precursor of prostaglandins (PGs), we investigated the effect of PGs on glucose consumption in 3T3-L1 cells. Among several PGs, only prostaglandin F 2alpha (PGF 2alpha) enhanced glucose consumption in 3T3-L1 cells treated with dexamethasone (DEX), 3-isobutyl-1-methyl-xanthine (IBMX), and insulin. To study the mechanism of PGF 2alpha-enhanced glucose consumption, we investigated the effect of PGF 2alpha on glycerol-3-phosphate dehydrogenase (GPDH) activity, triglycerides (TGs) content, and the expression of GLUT1 protein. PGF 2alpha suppressed GPDH activity and did not increase the expression of GLUT1 protein in 3T3-L1 cells treated with DEX, IBMX, and insulin. These results suggest that AA-stimulated glucose uptake is not through the effect of PGF 2alpha. Our results indicate that PGF 2alpha is a unique regulator of adipocyte differentiation (suppression) and glucose consumption (enhancement) in 3T3-L1 cells.