Membrane damage and enzyme inactivation of Lactobacillus plantarum by high pressure CO 2 treatment
Physiological changes of Lactobacillus plantarum (KFRI 815) by high pressure CO 2 treatment were investigated to examine the relevance to microbial inactivation. Characteristic properties of the cells measured in this study included salt tolerance, release of UV-absorbing substances, Mg and K ions,...
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Published in | International journal of food microbiology Vol. 63; no. 1; pp. 19 - 28 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
2001
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Subjects | |
Online Access | Get full text |
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Summary: | Physiological changes of
Lactobacillus plantarum (KFRI 815) by high pressure CO
2 treatment were investigated to examine the relevance to microbial inactivation. Characteristic properties of the cells measured in this study included salt tolerance, release of UV-absorbing substances, Mg and K ions, proton permeability, glycolysis, H
+-ATPase and constitutive enzymes, and dye uptake. The cells treated with high pressure CO
2 of 7 MPa at 30°C for 10 min showed the irreversible cellular membrane damages including loss of salt tolerance, leakage of UV-absorbing substances, release of intracellular ions, collapse of proton permeability and uptake of Phloxine B dye.
L.
plantarum cells after CO
2 treatment also exhibited reduced glycolytic activity and inactivation of some constituent enzymes. However, H
+-ATPase of the cell membrane maintained its initial specific activity of about 2.50 U/mg protein even though viability of the cells was reduced by several log cycles after high pressure CO
2 treatment. |
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ISSN: | 0168-1605 1879-3460 |
DOI: | 10.1016/S0168-1605(00)00393-7 |