Spectral analysis of the Ca 2+ concentration measurements in living cells

The effects of the experimental conditions and protein microenvironment on the fluorescence spectra of the Ca 2+-sensitive dye indo-1 in solutions and suspension of thymocytes have been studied. The temperature dependence of the contributions of L-, LM- and LP-forms of the dye in the integral fluore...

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Bibliographic Details
Published inJournal of molecular liquids Vol. 105; no. 2; pp. 131 - 136
Main Authors Zyma, V.L., Semenov, Yu.V., Prylutskyy, Yu.I., Miroshnichenko, M.S.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 2003
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Summary:The effects of the experimental conditions and protein microenvironment on the fluorescence spectra of the Ca 2+-sensitive dye indo-1 in solutions and suspension of thymocytes have been studied. The temperature dependence of the contributions of L-, LM- and LP-forms of the dye in the integral fluorescent spectrum of indo-1 were estimated. The changes of Ca 2+ concentration in thymocytes of the rat during activation evoked by high KCl solution were reported.
ISSN:0167-7322
1873-3166
DOI:10.1016/S0167-7322(03)00042-4