Spectral analysis of the Ca 2+ concentration measurements in living cells
The effects of the experimental conditions and protein microenvironment on the fluorescence spectra of the Ca 2+-sensitive dye indo-1 in solutions and suspension of thymocytes have been studied. The temperature dependence of the contributions of L-, LM- and LP-forms of the dye in the integral fluore...
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Published in | Journal of molecular liquids Vol. 105; no. 2; pp. 131 - 136 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
2003
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Online Access | Get full text |
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Summary: | The effects of the experimental conditions and protein microenvironment on the fluorescence spectra of the Ca
2+-sensitive dye indo-1 in solutions and suspension of thymocytes have been studied. The temperature dependence of the contributions of L-, LM- and LP-forms of the dye in the integral fluorescent spectrum of indo-1 were estimated. The changes of Ca
2+ concentration in thymocytes of the rat during activation evoked by high KCl solution were reported. |
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ISSN: | 0167-7322 1873-3166 |
DOI: | 10.1016/S0167-7322(03)00042-4 |