GABA A α1 subunit knock-out mice do not show a hyperlocomotor response following amphetamine or cocaine treatment

• Published in: Neuropharmacology Vol. 44; no. 2; pp. 190 - 198
• Main Authors: Reynolds, D.S., O’Meara, G.F., Newman, R.J., Bromidge, F.A., Atack, J.R., Whiting, P.J., Rosahl, T.W., Dawson, G.R.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2003
• Subjects: Diazepam; Dopamine; GABA; Knock-out; N-methyl- d-aspartate; α1; Dopamine; N-methyl- d-aspartate; GABA; Knock-out; Diazepam
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/S0028-3908(02)00370-2

The GABA A receptor system provides the major inhibitory control in the CNS, with the α1β2γ2 subunit combination being the most abundant and widely distributed form of the receptor. The α1 subunit knock-out (α1 KO) mice had a surprisingly mild overt phenotype, despite having lost ~60% of all GABA A receptors. The α1 KO mice had normal spontaneous locomotor activity, but were more sensitive to the sedating/ataxic effects of diazepam than wildtype (WT) mice. Pharmacological modulation of dopamine and N-methyl- d-aspartate (NMDA) receptors also produced altered responses in α1 KO mice compared with WT mice. As expected, the NMDA receptor antagonist MK801, amphetamine and cocaine increased locomotor activity in WT mice. Although MK801 increased locomotor activity in α1 KO mice, amphetamine and cocaine induced stereotypy not hyperlocomotion. Binding studies showed no gross changes in the total number of D1, D2 or NMDA receptors. Furthermore, pre-pulse inhibition of acoustic startle and the effects of cocaine in conditioned place preference were similar in both α1 KO and WT mice, indicating selective rather that global changes in response to dopaminergic agents. These data demonstrate subtle changes in behaviours mediated by neurotransmitters other than GABA in α1 KO mice and suggest that compensation may have occurred beyond the GABAergic system.