In vivo modulation of nucleus tractus solitarius (NTS) neurones by activation of 5-hydroxytryptamine 2 receptors in rats

• Published in: Neuropharmacology Vol. 39; no. 11; pp. 2006 - 2016
• Main Authors: Sévoz-Couche, Caroline, Wang, Y, Ramage, A.G, Spyer, K.M, Jordan, D
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2000
• Subjects: 5-HT 2 receptors; Brainstem; Ionophoresis; Rat; Vagal afferents; Ionophoresis; Rat; Brainstem; 5-HT 2 receptors; Vagal afferents
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/S0028-3908(00)00055-1

In in vivo experiments, 5-hydroxytryptamine (5-HT) and (±)-2,5-dimethoxy-4-iodoamphetamine HCl (DOI), a 5-HT 2 receptor agonist, were applied by ionophoresis to rat nucleus tractus solitarius (NTS) neurones identified by their vagal and cardiopulmonary afferent inputs to test whether the response of NTS cells to 5-HT 2 receptor activation was related to whether they received mono- or polysynaptic vagal inputs and their presumed function as defined by their afferent input. Cells were classified on the basis of the variability of the latency of the vagal-evoked spikes: this varied by less than 3 ms for Group 1, from 3 to 5 ms for Group 2, and more than 5 ms for Group 3. Both 5-HT and DOI inhibited most Group 1 cells (16/18) and inactive (without ongoing activity) cells (8/13) in Group 2. Cells inhibited by DOI were also inhibited by cardiopulmonary afferent stimulation, evoked by atrial phenylbiguanide administration. By contrast, application of 5-HT and DOI excited the majority of Group 3 cells (14/19) and Group 2 with ongoing activity (7/9). Cells excited by DOI were also activated by cardiopulmonary stimulation. Both actions of DOI were reversed by application of ketanserin ( n=15). In conclusion, these data demonstrate that activation of 5-HT 2 receptors in the NTS produces different effects dependent on whether the neurones received mono- or polysynaptic vagal input and their response to cardiopulmonary afferent stimulation.