Oxysterols induce apoptosis and accumulation of cell cycle at G 2/M phase in the human monocytic THP-1 cell line

The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols i...

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Published inLife sciences (1973) Vol. 72; no. 12; pp. 1389 - 1399
Main Authors Lim, Hee-Kyoung, Kang, Hee-Kyoung, Yoo, Eun-Sook, Kim, Bum-Joon, Kim, Youn-Woo, Cho, Moonjae, Lee, Jae-Hwa, Lee, Yun-Sil, Chung, Myung-Hee, Hyun, Jin-Won
Format Journal Article
LanguageEnglish
Published Elsevier Inc 2003
Subjects
Apoptosis
Cell cycle
Cytotoxicity
Oxysterol
Oxysterol
Cell cycle
Cytotoxicity
Apoptosis
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Summary:The cytotoxic activity of oxysterols, 7β-hydroxycholesterol (7β-OHC) and 25-hydroxycholesterol (25-OHC), has been evaluated using various leukemia cell lines. Among the tested cell lines, both oxysterols showed the highest cytotoxicity to THP-1, human monocytic leukemia cell line. These oxysterols induced apoptosis through down-regulation of Bcl-2 expression and activation of caspases. Also, the oxysterols showed the accumulation at G 2/M phase of cell cycle through down-regulation of cyclin B1 expression. Taken together, these results indicated that both 7β-OHC and 25-OHC inhibited the proliferation of THP-1 cells through apoptosis and cell cycle accumulation at G 2/M phase.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(02)02377-9