Modulatory action of PACAP 27 on NMDA receptor channel activity in cultured chick cortical neurons
The modulatory effect of PACAP 27 on NMDA receptor channel activity in cultured chick cortical neurons was investigated using the outside-out recording mode of the patch clamp technique. Channel opening frequency elicited by 20 μM NMDA, or 20 μM NMDA plus 1 μM glycine, was potentiated in the presenc...
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Published in | Brain research Vol. 791; no. 1; pp. 290 - 294 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
1998
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Subjects | |
Online Access | Get full text |
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Summary: | The modulatory effect of PACAP
27 on NMDA receptor channel activity in cultured chick cortical neurons was investigated using the outside-out recording mode of the patch clamp technique. Channel opening frequency elicited by 20
μM NMDA, or 20
μM NMDA plus 1
μM glycine, was potentiated in the presence of 100 nM PACAP
27 and inhibited with 1000 nM PACAP
27. These effects were reversible on washout and reduced when glycine concentration was increased to 10
μM, but were not affected by the PACAP antagonist PACAP
6-27 (1
μM) or the GTP inhibitor GDP-
β-S (100
μM). It is suggested that PACAP
27 may exert its modulatory action on NMDA receptor channel activity through the glycine site(s). |
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ISSN: | 0006-8993 1872-6240 |
DOI: | 10.1016/S0006-8993(97)01567-9 |