Modulatory action of PACAP 27 on NMDA receptor channel activity in cultured chick cortical neurons

The modulatory effect of PACAP 27 on NMDA receptor channel activity in cultured chick cortical neurons was investigated using the outside-out recording mode of the patch clamp technique. Channel opening frequency elicited by 20 μM NMDA, or 20 μM NMDA plus 1 μM glycine, was potentiated in the presenc...

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Bibliographic Details
Published inBrain research Vol. 791; no. 1; pp. 290 - 294
Main Authors Liu, Guo Jun, Madsen, Barry W
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1998
Subjects
Biphasic effect
Channel kinetics
Cross-reactivity
Glycine site
Neuromodulation
NMDA receptor
Patch clamp
Channel kinetics
Biphasic effect
Cross-reactivity
Patch clamp
NMDA receptor
Neuromodulation
Glycine site
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Summary:The modulatory effect of PACAP 27 on NMDA receptor channel activity in cultured chick cortical neurons was investigated using the outside-out recording mode of the patch clamp technique. Channel opening frequency elicited by 20 μM NMDA, or 20 μM NMDA plus 1 μM glycine, was potentiated in the presence of 100 nM PACAP 27 and inhibited with 1000 nM PACAP 27. These effects were reversible on washout and reduced when glycine concentration was increased to 10 μM, but were not affected by the PACAP antagonist PACAP 6-27 (1 μM) or the GTP inhibitor GDP- β-S (100 μM). It is suggested that PACAP 27 may exert its modulatory action on NMDA receptor channel activity through the glycine site(s).
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(97)01567-9