Prostaglandin D 2 in cultured capillary and microvascular endothelium of human brain
Production of prostaglandin D 2 (PGD 2) was investigated in cultured endothelial cells derived from capillaries and microvessels (small and large) of human brain using radioimmunoassays. Peptides, catecholamines, thrombin, protein kinase C-activating phorbol ester and calcium ionophore greatly stimu...
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Published in | Prostaglandins, leukotrienes and essential fatty acids Vol. 46; no. 3; pp. 231 - 234 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
1992
|
Online Access | Get full text |
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Summary: | Production of prostaglandin D
2 (PGD
2) was investigated in cultured endothelial cells derived from capillaries and microvessels (small and large) of human brain using radioimmunoassays. Peptides, catecholamines, thrombin, protein kinase C-activating phorbol ester and calcium ionophore greatly stimulated the secretion of endothelial PGD
2. Secretion of PGD
2 induced by vasoconstricting peptides, angiotensin II and arginine-vasopressin, was almost completely abolished by their respective specific receptor antagonists [Sar
1, Ala
8]-Ang II and [1−6(β-mercapto-β,β-cyclopentamethylene priopionic acid) 2-O-methyltyrosine]. Thus, the augmented production of PGD
2 by angiotensin II and arginine-vasopressin is a receptor-mediated event. It also indicates that the EC have specific angiotensin II and arginine-vasopressin (V
1) receptors. This study represents the first demonstration of vasoactive agents modulating PGD
2 production in capillary and microvascular endothelium of human brain. |
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ISSN: | 0952-3278 1532-2823 |
DOI: | 10.1016/0952-3278(92)90076-U |