Prostaglandin D 2 in cultured capillary and microvascular endothelium of human brain

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 46; no. 3; pp. 231 - 234
• Main Authors: Bacic, F., Uematsu, S., McCarron, R.M., Spatz, M.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 1992
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/0952-3278(92)90076-U

Production of prostaglandin D 2 (PGD 2) was investigated in cultured endothelial cells derived from capillaries and microvessels (small and large) of human brain using radioimmunoassays. Peptides, catecholamines, thrombin, protein kinase C-activating phorbol ester and calcium ionophore greatly stimulated the secretion of endothelial PGD 2. Secretion of PGD 2 induced by vasoconstricting peptides, angiotensin II and arginine-vasopressin, was almost completely abolished by their respective specific receptor antagonists [Sar 1, Ala 8]-Ang II and [1−6(β-mercapto-β,β-cyclopentamethylene priopionic acid) 2-O-methyltyrosine]. Thus, the augmented production of PGD 2 by angiotensin II and arginine-vasopressin is a receptor-mediated event. It also indicates that the EC have specific angiotensin II and arginine-vasopressin (V 1) receptors. This study represents the first demonstration of vasoactive agents modulating PGD 2 production in capillary and microvascular endothelium of human brain.