Acidic phospholipases A 2 from the venom of common sea snake enhydrina schistosa

Two acidic phospholipases A have been purified from the venom of common sea snake ( Enhydrina schistosa) by chromatography on Sephadex G-75 gel media, Bio-Rex 70 ion-exchanger followed by repeated Chromatography on DEAE-Sephadex A-25 ion-exchanger. The two preparations were shown to be homogeneous b...

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Bibliographic Details
Published inBiochimica et biophysica acta. General subjects Vol. 717; no. 3; pp. 503 - 508
Main Author Tan, Nget-Hong
Format Journal Article
LanguageEnglish
Published Elsevier B.V 1982
Subjects
Phospholipase A 2
Phospholipase A 2
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ISSN0304-4165
1872-8006
DOI10.1016/0304-4165(82)90294-X

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Summary:Two acidic phospholipases A have been purified from the venom of common sea snake ( Enhydrina schistosa) by chromatography on Sephadex G-75 gel media, Bio-Rex 70 ion-exchanger followed by repeated Chromatography on DEAE-Sephadex A-25 ion-exchanger. The two preparations were shown to be homogeneous by polyacrylamide gel electrophoresis and ion-exchange chromatography. The enzymes were shown to be specific for the ‘two’ position of egg yolk lecithin. The molecular weight of both enzymes determined by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis was approx. 14000. Both enzymes were non-lethal. Amino acid composition data indicated high contents of aspartic acid, glycine and alamine in both enzymes.
ISSN:0304-4165
1872-8006
DOI:10.1016/0304-4165(82)90294-X