Studies of the p 53 gene mutation in Saudi non-Hodgkin's lymphoma
Tumor biopsies (paraffin embedded tissue) obtained from 45 Saudi patients with non-Hodgkin's lymphoma (NHL) were examined for the incidence of p 53 mutations screened by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) analysis. DNA sequencing was carried out t...
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Published in | Cancer letters Vol. 104; no. 2; pp. 225 - 231 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ireland Ltd
1996
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Subjects | |
Online Access | Get full text |
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Summary: | Tumor biopsies (paraffin embedded tissue) obtained from 45 Saudi patients with non-Hodgkin's lymphoma (NHL) were examined for the incidence of p
53 mutations screened by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) analysis. DNA sequencing was carried out to confirm the occurrence of p
53 mutation in PCR products showing abnormal migration by SSCP analysis. Only
1
45
samples showed the incidence of a homozygous mutation at codon 179 (exon 5) of the p
53 gene that replaces histidine with tyrosine. The data showed that the frequency of p
53 mutations was very low in Saudi NHL. Our results are consistent with the general observation that the p
53 mutation is rather infrequent in hematopoietic malignancies like NHLs. |
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ISSN: | 0304-3835 1872-7980 |
DOI: | 10.1016/0304-3835(96)04255-3 |