Studies of the p 53 gene mutation in Saudi non-Hodgkin's lymphoma

• Published in: Cancer letters Vol. 104; no. 2; pp. 225 - 231
• Main Authors: Ahmad, Mir F., Nasrin, Nargis, Akhtar, Mohammed, Hannan, Mohammed A.
• Format: Journal Article
• Language: English
• Published: Elsevier Ireland Ltd 1996
• Subjects: Mutation; Non-Hodgkin's lymphoma; p 53 gene; Mutation; Non-Hodgkin's lymphoma; p 53 gene
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/0304-3835(96)04255-3

Tumor biopsies (paraffin embedded tissue) obtained from 45 Saudi patients with non-Hodgkin's lymphoma (NHL) were examined for the incidence of p 53 mutations screened by polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) analysis. DNA sequencing was carried out to confirm the occurrence of p 53 mutation in PCR products showing abnormal migration by SSCP analysis. Only 1 45 samples showed the incidence of a homozygous mutation at codon 179 (exon 5) of the p 53 gene that replaces histidine with tyrosine. The data showed that the frequency of p 53 mutations was very low in Saudi NHL. Our results are consistent with the general observation that the p 53 mutation is rather infrequent in hematopoietic malignancies like NHLs.