Collagenase activity in the human cervix uteri after prostaglandin E 2 application during the first trimester

Cervix biopsies were obtained during the first trimester from plurigravidae and primigravidae at various times after intracervical application of prostaglandin E 2. The tissues were extracted with Ca 2+-containing buffer, and collagenase activity was determined in these extracts using a solid phase...

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Bibliographic Details
Published inEuropean journal of obstetrics & gynecology and reproductive biology Vol. 42; no. 1; pp. 29 - 32
Main Authors Osmers, R., Rath, W., Adelmann-Grill, B.C., Fittkow, C., Szeverényi, M., Kuhn, W.
Format Journal Article
LanguageEnglish
Published Elsevier Ireland Ltd 1991
Subjects
Cervical ripening
Cervix uteri
Collagenase
First trimester
Prostaglandin E 2
Cervix uteri
First trimester
Collagenase
Cervical ripening
Prostaglandin E 2
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Summary:Cervix biopsies were obtained during the first trimester from plurigravidae and primigravidae at various times after intracervical application of prostaglandin E 2. The tissues were extracted with Ca 2+-containing buffer, and collagenase activity was determined in these extracts using a solid phase assay in which triple helical 125I-labelled collagen was cleaved. Collagenase was detected in all samples but elevated activity was present only during a short temporal window after prostaglandin application. Maximal activity was observed 1 and 2 h after application of prostaglandin in multigravidae and 4 h in primigravidae. These data can explain why collagenase activity in cervical tissues after prostaglandin application had previously not been found. They indicate somewhat different mechanisms of cervical ripening in primigravide compared to multigravidae.
ISSN:0301-2115
1872-7654
DOI:10.1016/0028-2243(91)90155-E