Regulation of histone H1 0 accumulation during induced differentiation of murine erythroleukemia cells

• Published in: Journal of molecular biology Vol. 217; no. 1; pp. 85 - 92
• Main Authors: Rousseau, Denis, Khochbin, Saadi, Gorka, Claude, Lawrence, Jean-Jacques
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 1991
• Subjects: MEL; HMBA; kb; GAPDH; PCA
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/0022-2836(91)90613-B

Histone H1 0 is one of the potential candidates that may contribute to the onset and stabilization of a genetic program during induced differentiation of murine erythroleukemia cells. In an attempt to understand better the role of H1 0 in this process we have tried to determine at which level the regulation of its induced accumulation occurs. Protein H1 0 was found to increase by a factor of 3 while its mRNA increased by a factor of 14, due to activation of gene transcription. As shown by H1 0 half-life measurements, the difference between the actual amount of H1 0 and that expected from the amount of mRNA was not due to increased turnover of the protein. Fractionation of the translational apparatus at several times during induction, revealed that H1 0 mRNA was efficiently transferred to the high molecular weight polysomes. The rate of synthesis of H1 0 was also increased by a factor of 4. Taken together, these results suggest the existence of a strong control at the translational level, which regulates H1 0 accumulation.