Differential translation of turnip yellow mosaic virus mRNAs [formula omitted]

Total TYMV RNA was incubated in a reticulocyte lysate, and the initiation peptides of the main proteins synthesized in vitro (195 K, 150 K and 20 K daltons) analyzed after tryptic digestion. The 195 K and the 150 K dalton proteins present analogous patterns, different from the one obtained with the...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 84; no. 4; pp. 831 - 839
Main Authors Benicourt, Claude, Haenni, Anne-Lise
Format Journal Article
LanguageEnglish
Published Elsevier Inc 1978
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Summary:Total TYMV RNA was incubated in a reticulocyte lysate, and the initiation peptides of the main proteins synthesized in vitro (195 K, 150 K and 20 K daltons) analyzed after tryptic digestion. The 195 K and the 150 K dalton proteins present analogous patterns, different from the one obtained with the 20 K dalton protein (coat protein), suggesting that only one initiation site exists on the genomic RNA for the synthesis of the two high molecular proteins. The results of competition experiments between genomic and coat protein mRNA indicate that the ribosomes have a much greater affinity for the coat protein mRNA. This may represent a regulatory mechanism for the preferential amplification of coat protein synthesis in the infected cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(78)91659-5