Reversal of the stereospecificity of the citrate synthase of [formula omitted] by p-chloromercuribenzoate

The stereospecificity of citrate synthase in extracts of Clostridium kluyveri (Barker strain) can be changed reversibly from the (R)-type to the (S)-type by treatment with p-chloromercuribenzoate. It is proposed that sulfhydryl groups are essential for the (R)-type stereospecificity of the enzyme an...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 34; no. 3; pp. 271 - 276
Main Authors O'Brien, R.W., Stern, Joseph R.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 1969
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Summary:The stereospecificity of citrate synthase in extracts of Clostridium kluyveri (Barker strain) can be changed reversibly from the (R)-type to the (S)-type by treatment with p-chloromercuribenzoate. It is proposed that sulfhydryl groups are essential for the (R)-type stereospecificity of the enzyme and their incomplete oxidation or reduction accounts for mixed labelling patterns in citrate.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(69)90827-4