The peracid cleavage of 5-methyltetrahydrofolic acid at the C 9N 10 bridge

5-Methyltetrahydrofolate cannot be cleaved at the C 9N 10 bond by the zinc/HCl reductive or the permanganate oxidative cleavage methods. A new method has been developed to perform this cleavage, using peracetic acid in 50% trifluoroacetic acid; the cleavage is quantitative and nondestructive of γ-g...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 92; no. 2; pp. 366 - 369
Main Authors Baugh, C.M., Braverman, E.B., Nair, M.G., Horne, D.W., Briggs, W.T., Wagner, C.
Format Journal Article
LanguageEnglish
Published Elsevier Inc 1979
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Summary:5-Methyltetrahydrofolate cannot be cleaved at the C 9N 10 bond by the zinc/HCl reductive or the permanganate oxidative cleavage methods. A new method has been developed to perform this cleavage, using peracetic acid in 50% trifluoroacetic acid; the cleavage is quantitative and nondestructive of γ-glutamyl peptide bonds.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(79)90672-9