Discrimination ofBordetella parapertussisandBordetella pertussisorganisms from clinical isolates by PCR using biotin-labelled oligonucleotide probes

• Published in: Molecular and cellular probes Vol. 12; no. 4; pp. 213 - 217
• Main Authors: Müller, F.-M.C, Heininger, U, Schnitzler, N, Kockelkorn, P, Cloot, O, Lorenz, C, Haase, G
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.1998
• Subjects: discriminationBordetella parapertussis/Bordetella pertussis, clinical isolates, specific oligonucleotide probes, pertussis vaccines; discriminationBordetella parapertussis/Bordetella pertussis, clinical isolates, specific oligonucleotide probes, pertussis vaccines
• ISSN: 0890-8508; 1096-1194
• DOI: 10.1006/mcpr.1998.0173

A recently developed shared-primer polymerase chain reaction (PCR) was investigated, in an ongoing pertussis surveillance study for discrimination ofBordetella parapertussisandBordetella pertussisorganisms, by using specific biotin-labelled oligonucleotide probes. From a total of 132 samples, 83 were positive by theB. parapertussisspecific probe, 33 were positive by theB. pertussisspecific probe and 16 samples containingHemophilus influenzaeas a negative control were below the threshold by both probes. The shared-primer PCR in combination with specific oligonucleotide probes provides a rapid, sensitive and specific molecular diagnostic tool for future surveillance studies. In addition, it may be used to further investigate whetherB. parapertussisantigens should be added to acellular pertussis vaccines to protect againstB. parapertussisinfections.