p16INK4andp15INK4BAlterations in Primary Gynecologic Malignancy

• Published in: Gynecologic oncology Vol. 65; no. 2; pp. 319 - 324
• Main Authors: Wong, Y.F., Chung, T.K.H., Cheung, T.H., Nobori, T., Yim, S.F., Lai, K.W.H., Yu, A.L., Diccianni, M.B., Li, T.Z., Chang, A.M.Z.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.05.1997
• ISSN: 0090-8258; 1095-6859
• DOI: 10.1006/gyno.1997.4669

Chromosome 9 abnormalities have been found in primary tumors and cell lines from human gynecologic malignancy. Alterations ofp16INK4andp15INK4Bgenes mapped on the band p21 of chromosome 9 have been detected in various human tumors, but the role of these genes as tumor suppressorsin vivoappear to be dependent on tumor type. Polymerase chain reaction (PCR)-based analysis was performed to search for lesions of these genes in 202 primary gynecologic malignancies. Homozygous deletions ofp16INK4were detected in 7 of 128 (5%) cervical, 1 of 41 (2%) endometrial, 2 of 27 (7%) ovarian, and 3 of 6 (50%) vulvar carcinomas, while homozygous deletions ofp15INK4Bwere detected in 19 of 128 (15%) cervical, 1 of 41 (2%) endometrial, 9 of 27 (33%) ovarian, and 3 of 6 (50%) vulvar carcinomas, respectively. No mutations were found in exon 2 ofp16INK4from 161 cases of gynecologic malignancy without deletion ofp16INK4. All 3 cases of vulvar carcinoma showing homozygous deletions ofp16INK4andp15INK4Bwere at advanced clinical stage (stage III–IV), while all 7 cases of cervical carcinoma and 2 cases of ovarian carcinoma showing homozygous deletion ofp16INK4were at early stage (stage I–II). The results indicate that homozygous deletions ofp16INK4and/orp15INK4Bgenes may play a role in a subset of primary gynecologic malignancy.