Microbial Synthesis and Characterization of Silver Nanoparticles Using Alternaria alternata

In this study, extracellular biosynthesis of silver nanoparticles was carried out using Alternaria alternata. Silver nanoparticles (AgNps) were synthesized using vegetative and cell free filtrate method. Silver nitrate (AgNO3) solution were reduced with mycelial mass and filtrate extracts in the rat...

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Bibliographic Details
Published inApplied environmental research Vol. 41; no. 1
Main Authors Sarafadeen O. Kareem, Oluwagbenga T. Familola, Adejare R. Oloyede, Enock O. Dare
Format Journal Article
LanguageEnglish
Published Environmental Research Institute, Chulalongkorn University 01.02.2019
Subjects
Alternaria alternata
Biosynthesis
Silver nanoparticles
TEM
XRD
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Summary:In this study, extracellular biosynthesis of silver nanoparticles was carried out using Alternaria alternata. Silver nanoparticles (AgNps) were synthesized using vegetative and cell free filtrate method. Silver nitrate (AgNO3) solution were reduced with mycelial mass and filtrate extracts in the ratio 1:4 v/v. Effects of biosynthesis parameters; time, temperature, light, darkness, static and agitation were also determined. Structural integrity of the silver nanoparticle was achieved using UV-visible spectrophotometry, X-ray diffraction (XRD) and Transmission Electron Microscope (TEM). Parametric optimization revealed that the cell free filtrate method had the highest intensity of 40 °C with constant agitation under darkness at 120 hr using 1mM AgNO3 concentration at maximum absorbance of 400 nm. TEM result showed that the AgNPs was homogeneous and well dispersed with spherical shape for A. alternata while XRD confirmed its purity giving AgNPs peaks. The nanoparticles were found to have the size ranging from 7.48–12.15 nm. This study presented Alternaria alternata as potential candidate for industrial biosynthesis of silver nanoparticles.
ISSN:2287-0741
2287-075X