The Study of Effects of Abscisic Acid (ABA) on Measure Serum Sex Hormones and Testis Tissue in Adult Male Rats

• Published in: Armaghān-i dānish Vol. 18; no. 7; pp. 578 - 589
• Main Authors: S Khatamsaz, P Heidari, M Shariati
• Format: Journal Article
• Language: Persian
• Published: Yasuj University Of Medical Sciences 01.11.2013
• Subjects: dihydrotestosterone; FSH; Key words: Abscisic acid; pituitary-gonadal axis; testosterone
• ISSN: 1728-6506; 1728-6514

Background & aim: Nowadays the effects of plant-based foods in maintaining and improving the health of many diseases have been considered. The aim of this study was to investigate the effect of Abscisic acid on serum sex hormone levels and testis of adult male rats, respectively. Methods: In this experimental study, 50 male Wistar rats were randomly divided into 5 groups: control, sham and experimental groups 1, 2 and 3 respectively. The control group received no drug treatment. The sham group received distilled water as a solvent. Groups 1, 2 and 3 were administered orally with 20, 400 and 800 mg /kg of extract containing plant hormone Abscisic acid for 40 days respectively. At the end of the fortieth day from all groups blood samples were taken and serum hormones LH, FSH, dihydrotestosterone, and testosterone were measured by ELISA. Testicular tissue changes were studied by optical microscopy after preparation of tissue samples and lineage sperm counts between experimental and control groups were studied. The data were analyzed by ANOVA and t-test. Results : Abscisic acid extract with doses of 400 and 800 mg/ kg at the end of the fortieth day significantly increased serum testosterone levels than the control group (p<0.05). No significant differences in serum hormones LH, FSH and DHT were observed in the experimental group compared to the control group (p<0.05). Primary spermatocytes, borderline Sertoli between experimental and control groups were not seen (p<0.05). Histology of seminiferous tubules and spermatogenesis cell also had not changed. Conclusion : Possibly during a period of 40-day the effects of Abscisic acid on receptor function , through the impact on N- methyl-D-aspartate receptors and γ receptor by proxysom ( PPARγ) and activation of LH receptors in Leydig cells lead to testes activation and cause increase in secretion of testosterone. Key words: Abscisic acid, testosterone, dihydrotestosterone, LH, FSH, pituitary-gonadal axis