Angelica sinensis polysaccharide antagonizes D-galactose induced brain aging in rats

Objective To explore the antagonistic effect of Angelica sinensis polysaccharide (ASP) on brain aging induced by D-galactose (D-gal) in rats. Methods SD rats were randomly divided into control group, ASP group, aging model group, and ASP aging model group. Their spatial learning and memory abilities...

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Published inDi 3 jun yi da xue xue bao Vol. 43; no. 19; pp. 1839 - 1845
Main Authors WANG Lan, ZHANG Mengsi, WANG Shunhe, WANG Ziling, HOU Jiying, XIAO Hanxianzhi
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Third Military Medical University 01.10.2021
Subjects
angelica sinensis polysaccharide
anti-aging
brain aging
d-galactose
neural stem cells
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Summary:Objective To explore the antagonistic effect of Angelica sinensis polysaccharide (ASP) on brain aging induced by D-galactose (D-gal) in rats. Methods SD rats were randomly divided into control group, ASP group, aging model group, and ASP aging model group. Their spatial learning and memory abilities were detected by water maze test. The aging of brain tissue cells were detectded by aging-related β-galactosidase (SA-β-Gal) staining. The contents of SOD, MDA and GSH in hippocampal homogenate were measured by chromatography, and those of IL-1β and IL-6 in hippocampal homogenate were detected by ELISA. Southern blotting and TRAP-PCR were used to detect telomere length and telomerase activity in the hippocampal cells. Neural stem cells (NSCs) were isolated, cultured and identified from the brain tissue of SD fetal rats. The third-generation NSCs were divided into control group, ASP group, aging model group, and ASP aging model group. The content of MDA in the cells was determined by chromatography. The intracellul
ISSN:1000-5404
DOI:10.16016/j.1000-5404.202103187