Liver X receptor agonist ameliorates amyloid β-induced inflammation and apoptosis in retinal pigment epithelial cells by activating SIRT1 signaling cascade

• Published in: Di 3 jun yi da xue xue bao Vol. 42; no. 14; pp. 1398 - 1406
• Main Authors: GAN Min, ZHOU Qiaoya, HONG Meijing, LI Xue, PENG Hui
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Third Military Medical University 01.07.2020
• Subjects: age-related macular degeneration; liver x receptor; retinal pigment epithelium; silencing information regulator 1; β-amyloid
• ISSN: 1000-5404
• DOI: 10.16016/j.1000-5404.202003092

Objective To investigate the role of liver X receptor (LXR)-SIRT1 regulatory axis in inflammation and apoptosis of retinal pigment epithelial (RPE) cells induced by β-amyloid (Aβ). Methods Human peripheral blood mononuclear cells (PBMCs) were collected from patients with wet age-related macular degeneration (AMD) and normal subjects for detection of SIRT1 mRNA expression. And then, human RPE cell line ARPE-19 stimulated with 1.0, 5.0 μmol/L Aβ1-40 or 5.0 μmol/L Aβ40-1 was examined for expression of SIRT1, IL-1β and p21 using real-time PCR to investigate the effects of Aβ on cell inflammation and apoptosis; Subsequently, the expression levels of SIRT1, LXRα, ace-NF-κB, NF-κB, ace-p53, p53, and p21 proteins in ARPE-19 cells treated with 5.0 μmol/L Aβ1-40, 5.0 μmol/L TO90, or both were detected using Western blotting to analyze the effect of Aβ1-40 stimulation and TO90 pretreatment on SIRT1 signaling cascade. Finally, after knocking down SIRT1, the expression of NF-κB and p53 signaling pathways was detected by W