Study of phytochemical, antioxidant, antimicrobial and anticancer activity of Berberis aristita

The present study was undertaken to find the phytochemical presence, antioxidant potential, antimicrobial activity, and anticancer activity of methanol extract of Berberis aristita. Plant extract showed the presence of antioxidants like alkaloid, saponin, terpenoids, coumarin, flavonoids, tannin, gl...

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Bibliographic Details
Published inJournal of Tropical Life Science Vol. 4; no. 1; pp. 001 - 007
Main Authors Basanta Lamichhane, Sandeep Adhikari, Pritish Shrestha, Bhupal Govinda Shrestha
Format Journal Article
LanguageEnglish
Published University of Brawijaya 01.10.2015
Subjects
Antioxidant
IC50
MTT
Phytochemicals
ZOI (Zone of Inhibition)
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Summary:The present study was undertaken to find the phytochemical presence, antioxidant potential, antimicrobial activity, and anticancer activity of methanol extract of Berberis aristita. Plant extract showed the presence of antioxidants like alkaloid, saponin, terpenoids, coumarin, flavonoids, tannin, glycoside and steroid. Further, antioxidant assay like DPPH (Diphenyl-2-picrylhydrazyl) assay, hydrogen peroxide assay and reducing power assay was done. DPPH free radical Scavenging Activity was expressed in % inhibition with L Ascorbic acid as standard showing IC50 9.6μg/ml and that of extract was 33.31μg/ml. Hydrogen peroxide radical scavenging activity was comparable to standard, IC50 for L Ascorbic acid is 54.23μg/ml and that of B. Aristita is 60.6μg/ml. Similarly, reducing power of plant extract at different concentration was comparable with L-Ascorbic Acid. Antimicrobial screening showed good positive result with Candida albicans, Salmonella typhii, Pseudomonas aeruginosa and Escherichia coli. Cell viability assay; MTT; showed a significant cytotoxicity to MDA-MB-231 and U-87 MG human cancer cell line compared to NIH/3T3 standard embryonic fibroblast cell lines of mouse.
ISSN:2087-5517