Expression and clinical significance of X-box binding protein 1 in peripheral blood B cells of patients with lupus nephritis

• Published in: Linchuang Shenzangbing Zazhi Vol. 24; no. 3; pp. 209 - 215
• Main Authors: Ming Yang, Ye-hua Fang
• Format: Journal Article
• Language: Chinese
• Published: Editorial Department of Journal of Clinical Nephrology 01.03.2024
• Subjects: endoplasmic reticulum stress; lupus nephritis; x-box binding protein 1
• ISSN: 1671-2390
• DOI: 10.3969/j.issn.1671-2390.2024.03.005

Objective To detect the expressions of X-box binding protein 1 unspliced （xbp1u） and X-box binding protein 1 spliced （xbp1s） mRNA in peripheral blood CD19+B cells of patients with lupus nephritis （LN） and healthy subjects and explore the clinical significance of XBP1 in LN patients. Methods The expression levels of xbp1u and xbp1s in peripheral blood CD19+B cells of 65 LN patients and 30 healthy controls were detected by real-time polymerase chain reaction （RT-PCR）. And the ratio of CD19−CD138+plasma cells/CD19+B cells in peripheral blood was detected by flow cytometry. Results The expression level of xbp1u mRNA in CD19+B cells of active LN group （0.078±0.034） was higher than that in control group（0.035±0.011）（P＜0.05） and in stable group（0.049±0.016）（P＜0.05）. The expression level of xbp1s mRNA in CD19+B cells of active LN group（0.076±0.037）was higher than that in control group（0.022±0.010）（P＜0.05） and stable group（0.043±0.017）（P＜0.05）. The ratio of CD19−CD138+ plasma cells /CD19+B cells in LN group（0.059±0.02