Bioinformatics analysis and experimental validation of ferroptosis in peri-implantitis
Objective To investigate the key genes associated with ferroptosis in peri-implantitis and explore the potential mechanisms regulating peri-implantitis. Methods Several datasets were obtained from the GEO database. Differential expressed genes were screened, and GO and KEGG analyses were performed....
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Published in | Kou qiang yi xue (Changzhou shi, China) Vol. 44; no. 7; pp. 527 - 535 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
Editorial Office of Stomatology
01.07.2024
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Subjects | |
Online Access | Get full text |
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Summary: | Objective To investigate the key genes associated with ferroptosis in peri-implantitis and explore the potential mechanisms regulating peri-implantitis. Methods Several datasets were obtained from the GEO database. Differential expressed genes were screened, and GO and KEGG analyses were performed. A PPI network was constructed using the STRING website. Key genes were validated using a test set, and the diagnostic value of key genes was determined. The content and proportion of 22 immune cells in peri-implantitis tissues were obtained through immune infiltration analysis. Key genes were validated by qRT-PCR and Western Blot(WB). Results There were 1 138 differential genes between peri-implantitis tissues and normal gingival tissues, of which 29 were related to ferroptosis. The gene expression in peri-implantitis tissues mainly involved processes such as immune response activation. Five key genes in the ferroptosis-related differential genes, namely SOX2, GJA1, IL1B, GPX2 and CHAC1, were differentially express |
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ISSN: | 1003-9872 |
DOI: | 10.13591/j.cnki.kqyx.2024.07.008 |