Bioinformatics analysis and experimental validation of ferroptosis in peri-implantitis

Objective To investigate the key genes associated with ferroptosis in peri-implantitis and explore the potential mechanisms regulating peri-implantitis. Methods Several datasets were obtained from the GEO database. Differential expressed genes were screened, and GO and KEGG analyses were performed....

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Published inKou qiang yi xue (Changzhou shi, China) Vol. 44; no. 7; pp. 527 - 535
Main Author ZHANG Zhewei, WANG Jiaohong, WU Wei, DONG Shuo, LI Guoqing, TANG Chunbo
Format Journal Article
LanguageChinese
Published Editorial Office of Stomatology 01.07.2024
Subjects
geo database|bioinformatics|peri-implantitis|ferroptosis|differential expressed genes
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Summary:Objective To investigate the key genes associated with ferroptosis in peri-implantitis and explore the potential mechanisms regulating peri-implantitis. Methods Several datasets were obtained from the GEO database. Differential expressed genes were screened, and GO and KEGG analyses were performed. A PPI network was constructed using the STRING website. Key genes were validated using a test set, and the diagnostic value of key genes was determined. The content and proportion of 22 immune cells in peri-implantitis tissues were obtained through immune infiltration analysis. Key genes were validated by qRT-PCR and Western Blot(WB). Results There were 1 138 differential genes between peri-implantitis tissues and normal gingival tissues, of which 29 were related to ferroptosis. The gene expression in peri-implantitis tissues mainly involved processes such as immune response activation. Five key genes in the ferroptosis-related differential genes, namely SOX2, GJA1, IL1B, GPX2 and CHAC1, were differentially express
ISSN:1003-9872
DOI:10.13591/j.cnki.kqyx.2024.07.008