Screening of differential lncRNAs and expression analysis of lncRNA ZEB2-AS1 in ovarian cancer tissues

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 42; no. 11; pp. 1675 - 1680
• Main Author: DONG Hui, WANG Ting-ting, MA Xiao-hong, MA Sheng-zong, DING Yong-hui
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.11.2022
• Subjects: ovarian cancer|lncrna|zinc finger e-box binding homeobox protein 2-as1(zeb2-as1)
• ISSN: 1001-6325
• DOI: 10.16352/j.issn.1001-6325.2022.11.1675

Objective To screen the valuable core(long non-coding RNAs,lncRNAs) from the tissue of ovarian cancer (OC) through expression profiling chip, and carry out expression verification and analysis. Methods Thirty-six tissue samples of ovarian cancer and of 22 normal ovaries were collected from January 2018 to June 2019 in the General Hospital of Ningxia Medical University and then expression profile chips were used to detect differentially expressed lncRNAs and mRNAs molecules, while real-time PCR was to verify the expression of lncRNAs. The expression of key core lncRNAs were measured by in situ hybridization, and the potential functions of lncRNAs were evaluated by bioinformatics. Results Compared with normal tissues, 4 982 different lncRNAs fragments were found in cancer tissues. The high expression of zinc finger E-box binding homeobox protein 2-AS1 (ZEB2-AS1) was the most significant one (P＜0.05); ZEB2-AS1 was over-expressed in multiple cancer tissues including liver cancer, colon cancer and kidney cancer;Th