Optimization of Deep Eutectic Solvents Extraction Process of Polysaccharides from Morchella septimelata and Probiotics Proliferation

• Published in: Shipin gongye ke-ji Vol. 46; no. 15; pp. 255 - 265
• Main Authors: Longying PEI, Li YANG, Sidan WANG, Xudong ZHAO, Xiaoyu WANG, Heng XU, Heng ZHANG, Yuting ZHOU, Wei LIU
• Format: Journal Article
• Language: Chinese
• Published: The editorial department of Science and Technology of Food Industry 01.08.2025
• Subjects: deep eutectic solvent; isolation and purification; morchella septimelata; polysaccharides extraction; proliferation of probiotics
• ISSN: 1002-0306
• DOI: 10.13386/j.issn1002-0306.202409115

The aim of this study was to develop a novel and efficient method for the extraction of polysaccharides from Morchella septimelata, to obtain highly purified Morchella septimelata polysaccharides and to assess their in vitro probiotic proliferative activities. In this study, the polysaccharides were extracted using deep eutectic solvents (DESs) to determine the optimal solvents composition and molar ratios, to optimize the polysaccharides extraction process through a one-way combination of a response surface test, and to examine the repeat extraction performance of the solvents. Additionally, the main components of the polysaccharides, in vitro proliferation of probiotics, and the acid-producing activity after purification were clarified. The results demonstrated that the optimal composition of DESs was choline chloride and citric acid (molar ratio 1:2), and that the optimal process for polysaccharide extraction was 44% water content, 62 ℃, 65 min and a 1:42 g/mL material-liquid ratio. The polysaccharide yiel