miR-205-5p inhibits the proliferation, migration and invasion of gastric cancer cell lines

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 40; no. 8; pp. 1068 - 1075
• Main Author: YANG Yi-li, HE Xue-yuan, PAN Xin-min, MA Jian-xun
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.08.2020
• Subjects: mir-205-5p|rap2b|gastric cancer|proliferation|migration
• ISSN: 1001-6325

Objective To investigate the effect of miR-205-5p on the proliferation, migration and invasion of gastric cancer cells by targeting at RAS oncogene family (RAP2B). Methods RT-qPCR and Western blot were used to detect the expression of miR-205-5p and RAP2B in gastric cancer cell lines AGS, MGC803, MKN-28, SGC-7901 and normal gastric mucosal cell line GES-1. Over expressing miR-205-5p, inhibition of RAP2B, cell viability was detected by MTT assay; cell migration and invasion ability were detected by Transwell method; The expression of RAP2B, cyclin D1, MMP-2, MMP-9 GSK-3β and β-catenin proteins were detected by Western blot. The dual luciferase reporter gene assay was used to verify the targeting of miR-205-5p to RAP2B. Results Compared with normal gastric mucosal cells, the expression of miR-205-5p was significantly decreased in four gastric cancer cell lines, and the expression of RAP2B was significantly increased (P＜0.05). The over-expression of miR-205-5p or inhibition of RAP2B inhibited the proliferation,