Effect of Scutellarin on Melanoma B16 in Vitro and in Vivo and Its Mechanism

The aim of the present study was to clarify the pathway and mechanism involved in the anti-melanona effect of Scutellarin (SCU). CCK-8 and Transwell assays were employed to evaluate the cell proliferation and migratory abilities of B16 in vitro. Then, a B16 tumor-bearing mouse model was established...

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Bibliographic Details
Published inShipin gongye ke-ji Vol. 44; no. 17; pp. 406 - 412
Main Authors Chongchong TIAN, Qi ZHANG, Xiaobo BAO, Yanshen CHEN
Format Journal Article
LanguageChinese
Published The editorial department of Science and Technology of Food Industry 01.09.2023
Subjects
angiogenesis
melanoma
migration and invasion
scutellarin
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Summary:The aim of the present study was to clarify the pathway and mechanism involved in the anti-melanona effect of Scutellarin (SCU). CCK-8 and Transwell assays were employed to evaluate the cell proliferation and migratory abilities of B16 in vitro. Then, a B16 tumor-bearing mouse model was established to explore the effect of SCU on tumor growth in vivo. Immunohistochemistry and flow cytometry were also used to examine the effect of SCU on tumor angiogenesis and the mobilization of bone marrow-derived cells, respectively. The results showed that SCU substantially decreased the proliferation of B16 cells at doses of 160, 320, and 640 μmol/L, respectively (P<0.01). Moreover, the migration and invasion capacity of B16 cells were apparently reduced when treated with 160 and 320 μmol/L SCU. Besides, the growth of B16 cells in vivo was remarably slower after administration of 30 and 60 mg/kg SCU (P<0.01). Further immunohistochemistry semi-quantitative results revealed the microvascular density (MVD) of B16 tumor tissu
ISSN:1002-0306
DOI:10.13386/j.issn1002-0306.2022110033