Efeito da Mannheimia granulomatis sobre cultivo de fibroblastos Effect of Mannheimia granulomatis on fibroblastos cultures

Primary cultures of Mannheimia granulomatis were established in chicken embryos to assess their capacity to stimulate fibroblast proliferation. The capacity of the bacterium-activated macrophages to stimulate cytokine and enzyme proliferation was assessed in a mouse peritoneum macrophage culture. To...

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Published inArquivo brasileiro de medicina veterinária e zootecnia Vol. 60; no. 2; pp. 509 - 512
Main Authors S. Ladeira, F.R. Gomes, T. Vidor, E.L. Portianski, E.J. Gimeno
Format Journal Article
LanguageEnglish
Published Universidade Federal de Minas Gerais 01.04.2008
Subjects
fibroblastos
fibroblasts
macrophages
macrófagos
Mannheimia granulomatis
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Summary:Primary cultures of Mannheimia granulomatis were established in chicken embryos to assess their capacity to stimulate fibroblast proliferation. The capacity of the bacterium-activated macrophages to stimulate cytokine and enzyme proliferation was assessed in a mouse peritoneum macrophage culture. To evaluate the bacteria infection on fibroblasts and their growth within 48h in relation to the active macrophages, cultures were washed and trypsinized and the cells counted. Results showed no significant differences when the bacteria-infected fibroblasts were mixed with bacterial extract (P=0.9682). The treatment using just products of macrophages resulted similar to the negative control. Significant differences on cell proliferation were established (P=0,0039) when the products of M. granulomatis-activated macrophages were used, meaning that bacterial components were unable to promote fibroblast increase. Further research is needed to elucidate the effect of M. granulomatis on the macrophages.
ISSN:0102-0935
1678-4162
DOI:10.1590/S0102-09352008000200038