Effects of Necdin on odontogenic differentiation and mineralization of rat dental germ ectodermal mesenchymal stem cells

Objective To investigate the regulative effect of neurally differentiated EC-cell-derived protein, Necdin, on the odontogenic differentiation and mineralization of rat dental germ ectomesenchymal stem cells (EMSCs). Methods Immunofluorescence staining was used to detect the expression and distributi...

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Published inLu jun jun yi da xue xue bao Vol. 45; no. 13; pp. 1413 - 1412
Main Authors ZHANG Yixin, YUAN Hongyan, LU Mingjie, XIE Bo
Format Journal Article
LanguageChinese
Published Editorial Office of Journal of Army Medical University 01.07.2023
Subjects
ectomesenchymal stem cells
mineralization
neurally differentiated ec-cell-derived protein
odontogenic differentiation
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Summary:Objective To investigate the regulative effect of neurally differentiated EC-cell-derived protein, Necdin, on the odontogenic differentiation and mineralization of rat dental germ ectomesenchymal stem cells (EMSCs). Methods Immunofluorescence staining was used to detect the expression and distribution of Necdin in E13.5d and E19.5d rat dental germ. EMSCs derived from E19.5d rat dental germ were isolated and cultured, and identified with flow cytometry for cell surface antigens. The EMSCs were stably transfected with lentiviral vector of Necdin silencing. Then, the apoptosis of EMSCs was detected by flow cytometry in the negative control group (sh-NC) and silenced group (sh-Necdin). The proliferation capacity of EMSCs was detected by CCK-8 assay, and the migration ability of EMSCs was detected by cell scratching assay. After mineralization induction, the regulative effect of Necdin on the odontogenic differentiation and mineralization of EMSCs was observed by alkaline phosphatase staining, alizarin red stainin
ISSN:2097-0927
DOI:10.16016/j.2097-0927.202303095