Recombinant globular adiponectin relieves kidney injury in diabetic mice

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 40; no. 4; pp. 518 - 522
• Main Author: YANG Li-li, GUO Zhi-xin, FENG Jin-yi, SONG Ying-lun, SHI Wen-jiao
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.04.2020
• Subjects: adiponectin|diabetic nephropathy|endoplasmic reticulum stress|autophagy
• ISSN: 1001-6325

Objective To investigate whether recombinant globular adiponectin can reduce kidney injury in diabetic mice by regulating autophagy and endoplasmic reticulum stress. Methods Mice were randomly divided into control group,diabetic model group, low and high dose of adiponectin group (5 and 15 μg/kg dose of recombinant globular adiponectin was injected). There were 8 mice in each group. After eight weeks, blood samples were collected to determine the level of blood glucose, urea nitrogen and creatinine by biochemical method and the level of insulin was measured by enzyme-linked immunosorbent assay. Bilateral kidneys were taken to calculate the ratio of the kidney mass to body mass. The renal cortex tissue samples were stained with HE, and the rest were kept at -70 ℃. The mRNA expression of endoplasmic reticulum stress indicators (GRP78, CHOP and caspase-12) and autophagy indicators (LC3, Beclin1 and p62) was determined by real-time quantitative PCR. Results Compared with control group, the level of blood glucose,