Effects and Mechanism of Resistin-like Molecules α on EndothelialCell Angiogenesis in Mouse Aorta

• Published in: Guoji laonian yixue zazhi Vol. 45; no. 1; pp. 41 - 45
• Main Authors: Shuxiang Duan, Haiyan Meng, Ping Sun, Ning Zhang, Hongmei Niu, Zhongsong Zhao, Haoyuan Li, Yonghui Ma
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of International Journal of Geriatrics 01.01.2024
• Subjects: angiogenesis; aorta; endothelial cells; mice; resistin-like molecules α
• ISSN: 1674-7593
• DOI: 10.3969/j.issn.1674-7593.2024.01.008

Objective To explore the effects and mechanism of resistin-like molecules α(also known as FIZZ1) on the ability of mouse aortic endothelial cells to form new blood vessels. Methods Mouse was stimulated by FIZZ1 at 10 nmol/L,20 nmol/L and 40 nmol/L.The role of FIZZ1 in endothelial cell migration and blood vessel formation was verified by using endothelial cell tube formation experiments and endothelial cell migration experiments in vitro;The effect of FIZZ1on signaling in mouse aortic endothelial cells was detected by high-throughput sequencing,and pathways with significant differential expression signaling were selected. Results Under different concentrations of FIZZ1,the area of blood vessel formation in mouse aortic endothelial cells gradually increased,and 40 nmol/L FIZZ1/ RELMα had the strongest effect in promoting angiogenesis.With the increasing concentration of FIZZ1,the number of endothelial cells migrated increased,and the migration capacity gradually increased.A total of 1 253 genes were found to be