Topical fish oil accelerates wound healing in rats with type 2 diabetes mellitus

• Published in: Lu jun jun yi da xue xue bao Vol. 46; no. 6; pp. 535 - 543
• Main Authors: HUANG Rongjiang, XIN Wenbin, NI Xiangmin
• Format: Journal Article
• Language: Chinese
• Published: Editorial Office of Journal of Army Medical University 01.03.2024
• Subjects: diabetic wounds; fish oil; inflammatory reactions; oxidative stress; silencing information regulator 1
• ISSN: 2097-0927
• DOI: 10.16016/j.2097-0927.202306068

Objective To observe the effect of topical fish oil on wound healing in rats with type 2 diabetes mellitus (T2DM) and to investigate its mechanism of action. Methods The T2DM rat model was established on 57 SD rats with a high-fat, high-sugar diet combined with intraperitoneal injection of STZ, and finally, T2DM model was established successfully in 49 rats. Then wound model was inflicted surgically, and the rats were divided into fish oil group (n=17), corn oil group (n=16) and model control group (n=16). Topical medication was applied to each group on the next day after wound modelling. During the intervention period, the wound healing was observed in all rats of each group. After the wound tissue samples were collected on 7 and 14 d after wound modelling, the levels of oxidative stress indexes (MDA, SOD and CAT) and inflammatory factors (TNF-α, IL-1β, IL-6 and IL-10) were detected, and pathological changes and collagen fibril deposition in the dermis were observed with HE and Masson staining. The expressio