Estudo espectroscópico do equilíbrio entre espécies hexacoordenada e pentacoordenada dos monômero d nativo e reconstituído da hemoglobina extracelular gigante de Glossoscolex paulistus em meio alcalino Spectroscopic study of the equilibrium between hexacoordinated and pentacoordinated species of the native and reconstituted d monomers of the Glossoscolex paulistus giant extracellular hemoglobin in alkaline medium

UV-Vis and fluorescence spectroscopic studies of native and reconstituted d monomers from Glossoscolex paulistus were performed in alkaline pH. In addition to the presence of aquomet and hemichrome hexacoordinated species, a pentacoordinated species occurs. This latter species, which presents an imi...

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Bibliographic Details
Published inQuímica nova Vol. 29; no. 4; pp. 666 - 673
Main Authors Julio C. Ribelatto, Alessandra L. Poli, Leonardo M. Moreira, Hidetake Imasato
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Química 01.07.2006
Subjects
extracellular hemoglobin
hexacoordinated
pentacoordinated
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Summary:UV-Vis and fluorescence spectroscopic studies of native and reconstituted d monomers from Glossoscolex paulistus were performed in alkaline pH. In addition to the presence of aquomet and hemichrome hexacoordinated species, a pentacoordinated species occurs. This latter species, which presents an imidazolate group of axial histidine as fifth ligand, is identified by the blue-shifted low-intensity Soret band and LMCT band. The pentacoordinated species must occur due to partial polypeptide unfolding.
ISSN:0100-4042
1678-7064
DOI:10.1590/S0100-40422006000400008