ASSOCIATION OF A POLYMORPHISM IN 1562 PROMOTER NUCLEOTIDE OF COLLAGENASES IV WITH THE AGE AND TYPE OF METASTASIS IN BREAST CANCER

• Published in: Majallah-i Dānishgāh-i ʻUlūm-i Pizishkī̄-i Bābul Vol. 10; no. 6; pp. 7 - 13
• Main Authors: M Sadeghi, M Motovali Bashi, Z Hojati
• Format: Journal Article
• Language: English
• Published: Babol University of Medical Sciences 01.01.2009
• Subjects: breast cancer; metastasis; polymorphism
• ISSN: 1561-4107; 2251-7170

BACKGROUND AND OBJECTIVE: Breast cancer is the most common malignancy among women and so leading cause of female death in middle age. So knowledge of the genes include in this disease such as collagenase-IV (MMP9) play important roles in cells segregation from the basic membrane and their metastasis. The aim of this study was to investigate the association between a functional polymorphism (C/T) at this gene promoter and its role in decrease of the age and so on location of metastasis in breast cancer patients.METHODS: This case-control study was accomplished in 90 subjects of breast cancer patients with metastasis and 100 health subjects. The follow-up and clinical tests were performed every two months. After genotyping subjects of this study were categorized into three types including CC, CT and TT and then relation between the genotype of every group was investigated with the type, age and location of metastasis. For this aim first the related primers was prepared and this location was reproduced by PCR technique then the genotype of each person was investigated by PCR products restriction enzyme digestion. FINDINGS: The results showed that from 90 subjects with metastasis, 37 subjects were under 47 years. Forty one subjects were with metastasis in lymphatic nodes and 21 subjects were with metastasis in wide bone. There was a significant association between the T allele exist and decrease of metastasis age under 47 years (OR= 7.31, 95% CI=2.4-21.8, p<0.001) and so the subject carrying this allele are two folds more susceptible for metastasis in wide bones in stead of lymphatic nodes.CONCLUSION: According to the results of this study, the T allele in collagenase-IV promoter is a risk factor for decrease the age of metastasis and also a risk for metastasis to wide bones is more than lymphatic nodes in breast cancer.