Establishment of CD36 negative platelet donor bank in Zhongshan area

• Published in: Zhongguo shu xue za zhi Vol. 35; no. 5; pp. 558 - 561
• Main Authors: Yonglun WU, Ainong SUN, fei PU, Qiao LI, Yuru FANG, Qianying CHEN, Yanting LIAO, Hongmei WANG, Yezhou CHEN, Shengbao DUAN
• Format: Journal Article
• Language: Chinese
• Published: Institute of Blood Transfusion of Chinese Academy of Medical Sciences 01.05.2022
• Subjects: blood donors; cd36 antigen; donor bank; platelet
• ISSN: 1004-549X
• DOI: 10.13303/j.cjbt.issn.1004-549x.2022.05.020

Objective To investigate the frequency of CD36 deletion and gene mutation in voluntary blood donors of Zhongshan city, and to explore the possibility of establishing local CD36 negative platelet donor bank. Methods Platelet CD36 antigen was detected by ELISA in 1 654 voluntary blood donors.Some of the negative samples were confirmed by flow cytometry, and genotyping was also performed. Results Platelet CD36 antigen was negative in 27 cases, accounting for 1.6% (27/1654), among which 1.6% (18/1149) were males and 1.8% (9/505) were females.No significant difference was noticed between males and females in CD36 antigen deletion cases (P>0.05). Fifteen CD36 negative samples were randomly selected, genotyped and sequenced, with type I deletion in 1 case[ 6.7% (1/15)], type Ⅱ deletion in 14 cases[ 93.3% (14/15)], and gene mutation in exon 3-14 detected in 8 cases. Conclusion The frequency of platelet CD36 antigen deletion in Zhongshan is comparable to that in other southern regions of China.The establishment of CD3