miR-486 is potentially associated with the occurrence of OSAHS comlicated with secondary polycythemia

• Published in: Ji chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 42; no. 8; pp. 1182 - 1187
• Main Author: SHI Xue-feng, SUN Ze-rui, HE Xiang, JIE You-bang, GU Yu-hai, DUO Jie
• Format: Journal Article
• Language: Chinese
• Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.08.2022
• Subjects: sleep apnea-hypopnea syndrome|secondary polycythemia|sirt1|gata-1
• ISSN: 1001-6325
• DOI: 10.16352/j.issn.1001-6325.2022.08.1182

Objective To explore the regulation and clinical significance of miR-486 in patients with sleep apnea-hypopnea syndrome (OSAHS) and secondary polycythemia. Methods Twenty-five healthy subjects who visited Outpatient Department and Health Examination Center of Qinghai Provincial People's Hospital from May 2020 to April 2021 were elected as the control group, and thirty cases of OSAHS patients with secondary polycythemia diagnosed by polysomnography and laboratory examination were selected as the OSAHS+SP group. Peripheral blood mononuclear cell (PBMC) miR-486, Sirt1 and GATA-1 expression were measured by RT-qPCR, and the concen- tration of plasma Sirt1 was measured by ELISA. In addition, RT-qPCR was used for the miR-486,Sirt1 or expression in K562 cells after the IH treatment, miR-486 over-expression or miR-486 inhibition. The Sirt1 expression was measured by RT-qPCR and Western blot. GATA-1 mRNA expression was checked by RT-qPCR in Sirt1 inhibited K562 cells. Results 1)Body mass index, red blood cells, hemogl