Optimization of Preparation Technology and in Vitro Digestion Analysis of Hydrolysates Origin from by-Products of Mandarin Fish

The by-products of mandarin fish were hydrolyzed with alcalase, while its optimized preparing process was performed with the single factor and response surface experiments, which were based on the degree of hydrolysis. Additionally, the in vitro digestion of prepared hydrolysates was further analyze...

Full description

Saved in:
Bibliographic Details
Published inShipin gongye ke-ji Vol. 43; no. 21; pp. 195 - 202
Main Authors Zhanchun WU, Ling LI, Yingqin ZHOU, Ningning XIE, Xubo FANG, Xiaoe CHEN
Format Journal Article
LanguageChinese
Published The editorial department of Science and Technology of Food Industry 01.11.2022
Subjects
antioxidant activity
by-products
hydrolysates
in vitro digestion
mandarin fish
Online AccessGet full text

Cover

More Information
Summary:The by-products of mandarin fish were hydrolyzed with alcalase, while its optimized preparing process was performed with the single factor and response surface experiments, which were based on the degree of hydrolysis. Additionally, the in vitro digestion of prepared hydrolysates was further analyzed. The results showed that: The optimum condition of the hydrolysis processing was that the ratio of solid-liquid was 1:4 g/mL, the pH was 9.1, the temperature was 57.0 ℃, the enzyme dosage was 2.02%, and the hydrolysis time was 4 h, with the degree of hydrolysis of which was 18.38%. During the simulated gastric digestion and the simulated intestinal digestion, the content of soluble peptides respectively decreased by 17.76% and 50.97%. Furthermore, after the whole gastrointestinal digestion, DPPH and ABTS radical scavenging rate, respectively, survived with values of 51.36% and 48.67%, which indicated that the prepared hydrolysates exerted relatively higher bioaccessibility. The results suggest that the by-product
ISSN:1002-0306
DOI:10.13386/j.issn1002-0306.2022010184