Efficacies of Tong-Xie-Yao-Fang Disassembled Recipes on Brain-gut Peptideina Rat Model of Diarrhea-predominant Irritable Bowel Syndrome

• Published in: Zhongguo quanke yixue Vol. 26; no. 5; pp. 569 - 575
• Main Author: WANG Kekai, YANG Yanlin, ZHOU Yanni, XIAO Jin, XIE Xin, WU Qinping, CHEN Min
• Format: Journal Article
• Language: Chinese
• Published: Chinese General Practice Publishing House Co., Ltd 01.02.2023
• Subjects: irritable bowel syndrome|diarrheatype irritable bowel syndrome|tong-xie-yao-fang|drugs, chinese herbal|visceral hypersensitivity|brain-derived neurotrophic factor|substance p
• ISSN: 1007-9572
• DOI: 10.12114/j.issn.1007-9572.2022.0510

Background Tong-Xie-Yao-Fang decoction has proven to be effective for irritable bowel syndrome (IBS) , a common intestinal disease. However, there are few studies on the main active ingredients in the decoction. Objective To examine the effects of Tong-Xie-Yao-Fang disassembled recipes on the expression levels of brain-derived neurotrophic factor (BDNF) and substance P (SP) in colon and hippocampus in a rat model of diarrhea-predominant irritable bowel syndrome (IBS-D) . Methods On July 2020, a total of 32 6-week-old SPF male Wistar rats were selected and randomized into blank group, model group, baishao-fangfeng group (B-F group) and chenpi-baizhu group (C-B group) , with 8 rats in each. Except for the blank group, the other three groups were used to establish visceral sensitive IBS-D rat model by use of colorectal dilatation + chronic restraint stress. At the end of successful model creation, the B-F group received the solution extracted from both baishao and fangfeng using gavage administration (4 ml/kg) ,