miR-493-5p inhibits the proliferation and migration of human ovarian cancer cell line SKOV3

Objective To investigate the effects of miR-493-5p on proliferation, invasion and migration of ovarian cancer cells and underlying mechanism. Methods Culture human normal ovarian epithelial cell lines IOSE80 and ovarian cancer cell lines A2780, SKOV3, OVCAR3, transfected the miR-493-5p over-expressi...

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Published inJi chu yi xue yu lin chuang = Jichu yixue yu linchuang = Basic medical sciences and clinics Vol. 41; no. 12; pp. 1749 - 1755
Main Author LI Shao-ru, QIN Hai-xia, LIU Shan-shan, HOU Rui-jie
Format Journal Article
LanguageChinese
Published Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College 01.12.2021
Subjects
mir-493-5p|git1|mek1/2-erk1/2 pathway|ovarian cancer|invasion
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Summary:Objective To investigate the effects of miR-493-5p on proliferation, invasion and migration of ovarian cancer cells and underlying mechanism. Methods Culture human normal ovarian epithelial cell lines IOSE80 and ovarian cancer cell lines A2780, SKOV3, OVCAR3, transfected the miR-493-5p over-expression vectors, miR-493-5p suppressed expression vectors and G protein coupled receptor kinase interacting factor 1 (GIT1) over-expression vectors into SKOV3 cells; RT-qPCR was used to detect miR-493-5p and GIT1 mRNA expression; Western blot was used to detect protein expression; MTT assay was used to detect cell viability; Transwell was used to detect cell migration and invasion; dual luciferase report experiment was applied for detection of the targeting relationship between miR-493-5p and GIT1. Results In ovarian cancer cells A2780, SKOV3, OVCAR3, miR-493-5p were weakly expressed, and GIT1 was highly expressed (P＜0.05); After over-expression of miR-493-5p, the proliferation activity, migration and invasion number of
ISSN:1001-6325