Research Progress in the Preparation and Applications of Hydrophilic Chromatographic Materials for Separation of Monosaccharides and Oligosaccharides

• Published in: Shípĭn kēxué Vol. 45; no. 9; pp. 261 - 271
• Main Author: ZHANG Dandan, JIAO Ruiwen, MI Chunxiao, CHEN Ju, LI Xiang, WANG Qiukuan, REN Dandan, HE Yunhai, WU Long, ZHOU Hui
• Format: Magazine Article
• Language: English
• Published: China Food Publishing Company 01.05.2024
• Subjects: monosaccharides; oligosaccharides; separation; chromatographic stationary phase; hydrophilic interaction chromatography
• ISSN: 1002-6630
• DOI: 10.7506/spkx1002-6630-20230329-285

Carbohydrates play a vital role in food science, medicine science, life science and other fields. Carbohydrates can be divided into monosaccharides, oligosaccharides, and polysaccharides, among which monosaccharides and oligosaccharides are mostly soluble, which can not only serve as structural substances, but also have various biological activities such as antioxidant, anticancer, antibacterial, and antiviral activities. Hydrophilic interaction chromatography makes up for the deficiency of reversed-phase liquid chromatography in the separation of carbohydrates. This paper reviews the stationary phases including amino bonded stationary phase, amide bonded stationary phase, carbohydrate bonded stationary phase and zwitterionic bonded stationary phase used in hydrophilic interaction chromatography for the separation and analysis of monosaccharides and oligosaccharides during 2017­–2022, and summarizes the effects of chromatographic separation conditions such as acetonitrile content, buffer salt concentration, mobile phase pH and column temperature on the hydrophilic separation efficiency so as to provide a reference for the separation and analysis of monosaccharides and oligosaccharides.