DNA polymorphism among mycelial compatibility groups of Sclerotinia sclerotiorum using ISSR marker

To assess the genetic structure of 38 Sclerotinia sclerotiorum isolates, representing 38 MCGs (Mycelial Comatibility Groups), collected from canola fields in Golestan, Mazandaran and Western Azarbaijan provinces, ISSR marker were used. Six out of 19 ISSR primers were reproducible and could amplify 2...

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Published inBiyutiknuluzhī-i kishāvarzī Vol. 2; no. 2; pp. 41 - 55
Main Authors Elham karimi, Nasser safaie, Masoud shamsbakhsh
Format Journal Article
LanguagePersian
Published Shahid Bahonar University of Kerman 01.11.2010
Subjects
discriminatory power
genetic diversity
issr
marker index
sclerotinia sclerotiorum
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Summary:To assess the genetic structure of 38 Sclerotinia sclerotiorum isolates, representing 38 MCGs (Mycelial Comatibility Groups), collected from canola fields in Golestan, Mazandaran and Western Azarbaijan provinces, ISSR marker were used. Six out of 19 ISSR primers were reproducible and could amplify 2124 bands ranging in size from approximately 120 bp to 3800 bp. ISSR09, ISSr10 and P1 primers had discriminatory power and marker index more than the others, indicating the efficiency of these three primers in discriminating of isolates. ISSR markers could differentiate 38 isolates into 36 genotypes, suggesting in S. sclerotiorum populations each MCG is genetically different from others.
ISSN:2228-6705
2228-6500
DOI:10.22103/jab.2012.368